鐜拌揣渚涘簲锛 NCI-H1688缁嗚優,浜虹粡鍏稿皬缁嗚優鑲虹檶缁嗚優璇存槑涔锛岃︾粏淇℃伅锛
缁嗚優鍚嶇О锛歂CI-H1688 鏆備笉渚涘簲浜虹粡鍏稿皬缁嗚優鑲虹檶缁嗚優
缁嗚優鎻忚堪锛氳繖鏍缁嗚優鏉ユ簮浜庝竴浣嶆不鐤椾箣鍓嶇殑鎮ｈ呯殑鑲濊浆绉荤伓銆
鍔ㄧ墿绉嶅埆锛氫汉
缁勭粐鏉ユ簮锛氳偤;鐧;缁忓吀灏忕粏鑳炶偤鐧
褰㈡侊細涓婄毊缁嗚優鏍
鍩瑰吇鍩哄拰娣诲姞鍓傦細RPMI-1640(GIBCO,璐у彿31800022, 娣诲姞NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)锛90%锛涗紭璐ㄨ儙鐗涜娓咃紝10%銆 姘旂浉锛氱┖姘旓紝95%锛涗簩姘у寲纰筹紝5%銆 娓╁害锛37鎽勬皬搴︺
REFERENCE锛欻ay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.

NCI-H1688缁嗚優,浜虹粡鍏稿皬缁嗚優鑲虹檶缁嗚優璇存槑涔鍩瑰吇鍩轰紶浠ｇ粏鑳炴搷浣滄ラ
(涓)鍘熺悊
浣撳栧煿鍏诲熀鐨勫師浠ｇ粏鑳炴垨缁嗚優鏍瑕佸湪浣撳栨寔缁鍦板煿鍏诲氨蹇呴』浼犱唬锛屼互渚胯幏寰楃ǔ瀹氱殑缁嗚優鏍鎴栧緱鍒板ぇ閲忕殑鍚岀嶇粏鑳烇紝骞剁淮鎸佺粏鑳炵嶇殑寤剁画銆傚煿鍏荤殑缁嗚優褰㈡垚鍗曞眰姹囧悎浠ュ悗锛岀敱浜庡瘑搴﹁繃澶х敓瀛樼┖闂翠笉瓒宠屽紩璧疯惀鍏绘灟绔锛屽皢鍩瑰吇鐨勭粏鑳炲垎鏁ｏ紝浠庡瑰櫒涓鍙栧嚭锛屼互1:2鎴杔:3浠ヤ笂鐨勬瘮鐜囪浆绉诲埌鍙﹀栫殑瀹瑰櫒涓杩涜屽煿鍏伙紝鍗充负浼犱唬鍩瑰吇銆
缁嗚優“涓浠”鎸囦粠缁嗚優鎺ョ嶅埌鍒嗙诲啀鍩瑰吇鐨勪竴娈垫湡闂达紝涓庣粏鑳炰笘浠ｆ垨鍊嶅炰笉鍚屻傚湪涓浠ｄ腑锛岀粏鑳炲煿澧3锝6娆°傜粏鑳炰紶浠ｅ悗锛屼竴鑸缁忚繃涓変釜闃舵碉細娓哥绘湡銆佹寚鏁板炵敓鏈熷拰鍋滄㈡湡銆
甯哥敤缁嗚優鍒嗚傛寚鏁拌〃绀虹粏鑳炲炴畺鐨勬椇鐩涚▼搴︼紝鍗崇粏鑳炵兢鐨勫垎瑁傜浉鏁帮紡100涓缁嗚優銆備竴鑸缁嗚優鍒嗚傛寚鏁颁粙浜0.2锛咃綖0.5锛咃紝鑲跨槫缁嗚優鍙杈3锝5锛呫傜粏鑳炴帴绉2锝3澶╁垎瑁傚炴畺鏃虹洓锛屾槸娲诲姏鏈濂芥椂鏈燂紝绉版寚鏁板炵敓鏈(瀵规暟鐢熼暱鏈)锛岄傚疁杩涜屽悇绉嶈瘯楠屻
(浜)鎿嶄綔
1锛庡皢闀挎垚鍗曞眰鐨勫師浠ｅ煿鍏荤粏鑳炴垨HeLa缁嗚優浠庝簩姘у寲纰冲煿鍏荤变腑鍙栧嚭锛屽湪瓒呭噣宸ヤ綔鍙颁腑鍊掓帀鐡跺唴鐨勫煿鍏诲熀娑诧紝鍔犲叆灏戣告秷鍖栨恫銆(浠ユ恫闈㈢洊浣忕粏鑳炰负瀹)锛岄潤缃5锝10鍒嗛挓銆
2锛庡湪鍊掔疆闀滀笅瑙傚療琚娑堝寲鐨勭粏鑳烇紝濡傛灉缁嗚優鍙樺洯锛岀浉浜掍箣闂翠笉鍐嶈繛鎺ユ垚鐗囷紝杩欐椂搴旂珛鍗冲湪瓒呭噣鍙颁腑灏嗘秷鍖栨恫鍊掓帀锛屽姞鍏3锝5ml鏂伴矞鍩瑰吇娑诧紝鍚规墦锛屽埗鎴愮粏鑳炴偓娑层
3锛庡皢缁嗚優鎮娑插惛鍑2ml宸﹀彸锛屽姞鍒板彟涓涓鍩瑰吇鐡朵腑骞跺悜姣忎釜鐡朵腑鍒嗗埆鍔3ml宸﹀彸鍩瑰吇娑诧紝鐩栧ソ鐡跺烇紝閫佸洖浜屾哀鍖栫⒊鍩瑰吇绠变腑锛岀户缁杩涜屽煿鍏汇
(涓)缁撴灉
涓鑸鎯呭喌锛屼紶浠ｅ悗鐨勭粏鑳炲湪2灏忔椂宸﹀彸灏辫兘闄勭潃鍦ㄥ煿鍏荤摱澹佷笂锛2锝4澶╁氨鍙鍦ㄧ摱鍐呭舰鎴愬崟灞傦紝闇瑕佸啀娆¤繘琛屼紶浠ｃ

NCI-H1688缁嗚優,浜虹粡鍏稿皬缁嗚優鑲虹檶缁嗚優璇存槑涔鏈夌幇璐э紝鍏鍙稿叾浠栫粏鑳炰骇鍝侊細

YMK0870 FO 缁嗚優锛 灏忛紶楠ㄩ珦鐦ょ粏鑳 FO 缁嗚優
YMK0880 FRhK-4 缁嗚優锛 鎭掓渤鐚磋儦鑲剧粏鑳 FRhK-4 缁嗚優
YMK0890 GBC-SD 缁嗚優锛 鑳嗗泭鐧岀粏鑳 GBC-SD 缁嗚優
YMK0900 H9c2(2-1) 缁嗚優锛 澶ч紶蹇冭倢缁嗚優 H9c2(2-1) 缁嗚優
YMK0910 HA 缁嗚優锛 缇婅啘缁嗚優 HA 缁嗚優
YMK0920 HBL-100 缁嗚優锛 鏁村悎SV40 鍩哄洜鐨勪钩xian涓婄毊缁嗚優 HBL-100 缁嗚優
YMK0930 HCC 94 [HCC941122] 缁嗚優锛 瀛愬槌炵檶缁嗚優锛堥珮鍒嗗寲锛 HCC 94 [HCC941122] 缁嗚優
YMK0940 HCC1937缁嗚優锛 涔硏ian鐧岀粏鑳 HCC1937缁嗚優
YMK0950 HCC827缁嗚優锛 闈炲皬缁嗚優鑲虹檶缁嗚優 HCC827缁嗚優
YMK0960 HCCC-9810 缁嗚優锛 鑳嗙＄粏鑳炲瀷鑲濈檶缁嗚優 HCCC-9810 缁嗚優
YMK0970 Hce-8693 缁嗚優锛 鐩茶偁鑵虹檶缁嗚優锛堟湭鍒嗗寲锛 Hce-8693 缁嗚優
YMK0980 HCT 116 缁嗚優锛 缁撹偁鐧岀粏鑳 HCT 116 缁嗚優
YMK0990 HCT-15 缁嗚優锛 缁撶洿鑲犺吅鐧岀粏鑳 HCT-15 缁嗚優
YMK1000 HCT-8 [HRT-18] 缁嗚優锛 鍥炵洸鑲犵檶缁嗚優 HCT-8 [HRT-18] 缁嗚優
YMK1010 HEC-1-A缁嗚優锛 瀛愬鍐呰啘鑵虹檶缁嗚優 HEC-1-A缁嗚優
YMK1020 HEC-1-B 缁嗚優锛 瀛愬鍐呰啘鑵虹檶缁嗚優 HEC-1-B 缁嗚優
YMK1030 HEL 缁嗚優锛 绾㈢櫧缁嗚優鐧借鐥呯粏鑳 HEL 缁嗚優
YMK1040 HeLa 缁嗚優锛 瀹玧ing鐧岀粏鑳 HeLa 缁嗚優
YMK1050 HeLa 229 缁嗚優锛 瀹玧ing鐧岀粏鑳 HeLa 229 缁嗚優

YMS11425B 婧磋劚姘ф牳鑻峰翱鍢у暥(BrdU)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human voltage-gated calcium channel,VGCC Elisa璇曞墏鐩
YMS11426B 鑳冪矘娑茬礌(GM)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Bromodeoxyuridine,BrdU Elisa璇曞墏鐩
YMS11427B 绾ょ淮姣嶇粏鑳炶〃闈㈡姉鍘(FSP)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Gastric Mucin,GM Elisa璇曞墏鐩
YMS11428B 杞绉诲洜瀛(TF)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Fibroblast surface protein,FSP Elisa璇曞墏鐩
YMS11429B 楦熸皑閰歌劚缇ч叾(ODC)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Transfer factor,TF Elisa璇曞墏鐩
YMS11430B 榛戣壊绱犵槫鐩稿叧鎶楀師(MAGE)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Ornithine decarboxylase,ODC Elisa璇曞墏鐩
YMS11431B 鍓嶈兌鍘烠绔鑲介叾(PCP)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human melanoma-associated antigen,MAGE Elisa璇曞墏鐩
YMS11432B 澶氬舰鏍哥櫧缁嗚優寮规ц泲鐧介叾(PMN Elastase)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human procollagen C peptidase,PCP Elisa璇曞墏鐩
YMS11433B 琛ヤ綋鐗囨柇3b(C3b)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Polymorphonuclear Elastase,PMN Elastase Elisa璇曞墏鐩
YMS11434B 琛ヤ綋鐗囨柇4b(C4b)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Complement fragment 3b,C3b Elisa璇曞墏鐩
YMS11435B 琛ヤ綋鐗囨柇5b(C5b)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Complement fragment 4b,C4b Elisa璇曞墏鐩
YMS11436B 涓嶅圭О浜岀敳鍩虹簿姘ㄩ吀(ADMA)ELISA璇存槑涔﹁瘯鍓傜洅锛96T/48T璇存槑涔 Human Complement fragment 5b,C5b Elisa璇曞墏鐩

NCI-H1688缁嗚優,浜虹粡鍏稿皬缁嗚優鑲虹檶缁嗚優璇存槑涔涓婃捣鐮旂洘鐢熺墿绉戞妧鏈夐檺鍏鍙镐富瑕佷骇鍝佹秹鍙婏細ELISA璇曞墏鐩掞紝鐢熺墿璇曞墏锛岃娓咃紝鎶椾綋锛屾爣鍑嗗搧锛屽煿鍏诲熀绛夌戠爺浜у搧锛屾㈣繋鏂拌佸㈡埛鏉ョ數鍜ㄨ銆